In vivo 3D mapping of intracellular pH using hyperpolarized [1-13C]pyruvate in the rodent heart
Angus Zoen Lau1,2, Jack Miller2,3, and Damian J Tyler1,2
1Cardiovascular Medicine, University of Oxford, Oxford, United Kingdom, 2Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom, 3Department of Physics, Clarendon Laboratory, Oxford, United Kingdom
Intracellular pH can be measured in vivo by taking the ratio between hyperpolarized HCO3-
and CO2 produced from [1-13C]pyruvate, but to date, low
CO2 signal has limited this method to whole-heart assessment. We
propose a 3D imaging approach using spectrally-selective excitation which
exploits the rapid exchange between HCO3- and CO2
to produce in vivo myocardial pHi maps. The intracellular pHi
is found to be 7.15±0.04 in the healthy rodent heart. Increased cardiac
workload via continuous dobutamine infusion resulted in a decreased intracellular
pH of 6.90±0.06.
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