Abstract #3669

In vivo 3D mapping of intracellular pH using hyperpolarized [1-13C]pyruvate in the rodent heart

Angus Zoen Lau^1,2, Jack Miller^2,3, and Damian J Tyler^1,2

¹Cardiovascular Medicine, University of Oxford, Oxford, United Kingdom, ²Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom, ³Department of Physics, Clarendon Laboratory, Oxford, United Kingdom

Intracellular pH can be measured in vivo by taking the ratio between hyperpolarized HCO₃^- and CO₂ produced from [1-¹³C]pyruvate, but to date, low CO₂ signal has limited this method to whole-heart assessment. We propose a 3D imaging approach using spectrally-selective excitation which exploits the rapid exchange between HCO₃^- and CO₂ to produce in vivo myocardial pH_i maps. The intracellular pH_i is found to be 7.15±0.04 in the healthy rodent heart. Increased cardiac workload via continuous dobutamine infusion resulted in a decreased intracellular pH of 6.90±0.06.

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