Abstract #3958

Citrate production in prostate cancer metastasis cell lines LNCaP and VCaP

Frits H.A. van Heijster¹, Vincent Breukels¹, Kees (C). J. Jansen², Jack A. Schalken², and Arend Heerschap¹

¹Radiology and Nuclear Medicine, Radboud University Medical Center, Nijmegen, Netherlands, ²Urology, Radboud University Medical Center, Nijmegen, Netherlands

Citrate production by prostate cancer cell lines LNCaP and VCaP were studied with ¹³C-NMR spectroscopy. Glucose acts as carbon source for citrate production in LNCaP and VCaP cell lines, but not aspartate, in contrast to expectation. Also pyruvate and glutamine can act as carbon sources for citrate production in LNCaP. Anaplerosis via pyruvate carboxylase is found to be low in these cells. Glutamine label only ends up in citrate via isocitrate instead of downstream in the Krebs cycle. These typical features of citrate metabolism might serve as valuable biomarkers for transition from healthy prostate cells to malignant cells.

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