Abstract #4166

Neurobiological Quantification of Stress-Induced Sleep-Perturbation in Rats using In Vivo Proton MR Spectroscopy and In Vitro Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Do-Wan Lee^1,2, Seockhoon Chung³, Hyun Ju Yoo⁴, Su Jung Kim⁴, Chul-Woong Woo², Sang-Tae Kim², Kyungwon Kim⁵, Jeong-Kon Kim⁵, Jin Seong Lee⁵, Choong Gon Choi⁵, Woo Hyun Shim⁵, Dong-Hoon Lee¹, Yoonseok Choi², and Dong-Cheol Woo²

¹Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ²MR Core Laboratory, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea, Republic of, ³Department of Psychiatry, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Republic of, ⁴Biomedical Research Center, Asan Institute for Life Sciences, Asan Medical Center University of Ulsan College of Medicine, Seoul, Korea, Republic of, ⁵Department of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Republic of

The aim of this study was to quantitatively assess the differences on the cerebral metabolites and to identify factors determining the alterations of endogenous biomolecules on stress-induced sleep disturbance in rats using in vivo 1H-MRS and in vitro LC-MS/MS. The GABA, Gln concentrations, Gln/Glu, Gln/tCr, and GABA/Glu ratios were significantly higher in SSP rats than in CNTLs. The serotonin concentrations were significantly lower in SSP rats than in CNTLs. Our in vivo 1H MRS and in vitro LC-MS/MS results suggest that the various metabolites and endogenous biomolecule signals in hippocampal region are particularly sensitive and vulnerable to stress-induced sleep perturbation.

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