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Abstract #4341

2HG Optimized PRESS MRS for Metabolic Profiling of IDH-mutant Gliomas in Patient-Derived Orthotropic Mouse Model of Human Brain Tumor

Vivek Tiwari1, Tomoyuki Mashimo2, Sandeep Kumar Ganji1, Zhongxu An1, Keith Hulsey3, Vamsidhara Vemireddy2, Shanrong Zhang1, Elizabeth Maher2, Robert Bachoo2, and Changho Choi1

1Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas, TX, United States, 2Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX, United States, 3Department of Radiology, UT Southwestern Medical Center, Dallas, TX, United States

2-Hydroxyglutarate (2HG) is elevated in gliomas harboring IDH1 or IDH2 mutations, and can be a biomarker for the diagnosis of IDH-mutant gliomas. The present study was undertaken to develop PRESS MRS Methodology for 2HG-detection with enhanced precision, and studying 2HG kinetics together with metabolic profiling in Patient-Derived Xenograft Orthotropic Mouse model. In-vivo PRESS MRS was performed at an optimized long-TE of 96ms. A large inverted-signal of 2HG at 2.25-ppm was obtained from IDH-mutant gliomas, well separated from neighboring signals of positive-polarity, with minimal interference from macromolecules. The method was successfully employed to detect 2HG and brain metabolism with tumor progression.

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