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Abstract #0032

Direct Quantitative 13C-Filtered 1H Magnetic Resonance Imaging of PEGylated Biomacromolecules In Vivo

Rohan D. A. Alvares1, Justin Y. Lau2,3, Peter M. Macdonald1, Charles H. Cunningham2,3, and R. Scott Prosser1,4

1Department of Chemistry, University of Toronto, Toronto, ON, Canada, 2Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada, 3Sunnybrook Research Institute, Toronto, ON, Canada, 4Department of Biochemistry, University of Toronto, Toronto, ON, Canada

We demonstrate a new platform technology in which macromolecular constituents, such as proteins and drug delivery systems, are observed directly and quantitatively in vivo using 1H MRI of 13C-labeled polyethylene glycol (13C-PEG) tags. The 28 kDa 13C-PEG tags are non-immunogenic, and each bears approximately 2500 spectroscopically equivalent 1H nuclei appearing at a single resonance position. By filtering the 1H PEG signal through the directly coupled 13C nuclei, background water and fat signals are largely eliminated. We demonstrate the approach by monitoring in real-time the distribution of 13C-PEG and 13C-pegylated albumin injected into the hind leg of a mouse.

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