NMR allows to investigate multiple aspects of physiological parameters like regional perfusion, blood and tissue oxygenation, intracellular pH or high-energy phosphate metabolism. In the past, interleaved multi-parametric multi-nuclear dynamic NMR imaging and spectroscopy of skeletal muscle was developed on prototype scanners. Here we developed an interleaved pulse sequence combining NMR acquisitions of a perfusion image, 1H deoxy-myoglobin and 31P spectra on a clinical system without any hardware modifications from the customer. We successfully evaluated this sequence in the ischemic calf muscle and exercising quadriceps muscle. Nevertheless, using a surface coil for pulsed-ASL measurements remains a limitation at this time.