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Abstract #0114

Measuring temperature in brown adipose tissue using the proton chemical shift

Clemens Diwoky1, Renate Schreiber1, and Rudolf Zechner1

1Institute of Molecular Biosciences, University of Graz, Graz, Austria

Within this work an approach based on the temperature dependence of the proton resonance frequency (PRF) of water and methylene bound protons is followed to monitor in-vivo thermogenesis in the interscapular brown adipose tissue (iBAT) of mice. Measuring the change in chemical shift difference of water and fat rather than water alone, known problems of PRF-based temperature measurements such as magnetic field drift and the temperature dependence of magnetic susceptibility are circumvented. The study determines the temperature coefficient in ex-vivo iBAT tissue extracts and presents the application of the technique in measuring norepinephrine stimulated in-vivo iBAT thermogenesis.

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