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Abstract #0124

In Vivo Biochemical and Histological Validation of Proton Density Fat Fraction as a Quantitative Biomarker of Hepatic Steatosis

Scott B Reeder1,2,3,4,5, Curtis N Wiens1, Nathan Artz1,6, Jeffrey B Schwimmer7, Rashmi Agni8, Rao Watson8, Tanya Wolfson9, Anthony Gamst10, Guilherme Campos11,12, Santiago Horgan13, Luke Funk12, Garth Jacobsen13, Jacob Greenberg12, Alexandra Schlein14, Yesenia Covarrubias14, Jonathan C Hooker14, Michael S Middleton14, Gavin Hamilton14, Benjamin Ratliff1,3, Alan B McMillan1, Diego Hernando1,2, and Claude B Sirlin14

1Radiology, University of Wisconsin, Madison, WI, United States, 2Medical Physics, University of Wisconsin, Madison, WI, 3Biomedical Engineering, University of Wisconsin, Madison, WI, United States, 4Medicine, University of Wisconsin, Madison, WI, United States, 5Emergency Medicine, University of Wisconsin, Madison, WI, United States, 6Diagnostic Imaging, St. Jude Children’s Research Hospital, 7Pediatrics, University of California, San Diego, 8Pathology, University of Wisconsin, 9San Diego Super Computer Center, University of California, San Diego, 10Mathematics, University of California, San Diego, 11Surgery, Virginia Commonwealth University, Richmond, VA, United States, 12Surgery, University of Wisconsin, Madison, WI, 13Surgery, University of California, San Diego, San Diego, CA, United States, 14Radiology, University of California, San Diego

The purpose of this study was to validate complex- and magnitude-based quantitative chemical-shift encoded MRI (CSE-MRI) to quantify PDFF as an accurate biomarker of hepatic steatosis. A biopsy-MRI correlation study was performed in 95 obese subjects undergoing bariatric weight loss surgery. The results of biochemical triglyceride, conventional histological, digital histological, and MR spectroscopy analyses were used as reference standards. Strong correlations were found between MRI-PDFF measured using both complex- and magnitude-based CSE-MRI methods vs. each reference standard. This is the first cross-sectional in-vivo study validating PDFF as a biomarker of hepatic steatosis using biochemical triglyceride concentration as the reference.

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