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Abstract #0166

Effects of 3-MPA on in vivo hepatic metabolism of hyperpolarized [1-13C] pyruvate

Emine Can1, Hikari A.I. Yoshihara1,2, Jessica A.M. Bastiaansen1,2, Rolf Gruetter3,4, and Arnaud Comment1

1Institute of Physics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, 2Division of Cardiology, University Hospital Lausanne (CHUV), Lausanne, Switzerland, 3Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, 4Department of Radiology, University of Lausanne (UNIL), Lausanne, Switzerland

Ex vivo and in vivo studies on liver metabolism using hyperpolarized [1-13C]pyruvate report do not agree on whether hyperpolarized bicarbonate metabolite production results from pyruvate oxidation or gluconeogenesis. This study tested the ability of hyperpolarized [1-13C]pyruvate to probe gluconeogenesis in the liver of intact rats. While conversion to hyperpolarized bicarbonate was detected in the liver of fasted rats, treatment with the phosphoenolpyruvate carboxykinase inhibitor 3-mercaptopicolinc acid resulted in 7-fold lower levels. This result supports the notion that hepatic gluconeogenic metabolism can indeed be directly probed in vivo with hyperpolarized pyruvate.

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