Abstract #0312

Fluorine-19 NMR cytometry to quantify human transgenic CAR T cell biodistribution in murine studies of glioblastoma immunotherapy

Fanny Chapelin¹, Hideho Okada², and Eric T Ahrens³

¹Bioengineering, University of California San Diego, La Jolla, CA, United States, ²Neurological surgery, University of California San Francisco, San Francisco, CA, United States, ³Radiology, University of California San Diego, La Jolla, CA, United States

Technologies to quantify the biodistribution of emerging immunotherapeutic cell therapies against cancer can accelerate the timeline to evaluate potential candidates. In this study, we describe the use of in ‘NMR cytometry’ to assay immunotherapeutic cell biodistribution in a mouse model of sub-cutaneous glioblastoma (U87) treated with chimeric antigen receptor (CAR) T-cells. We examine CAR T cell ¹⁹F labeling efficiency, phenotype and biodistribution with ¹⁹F NMR at day 2 and 7 post infusion to elucidate T-cell tumor homing, survival, and tissue distribution.

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