The acoustic radiation (AR) is a compact bundle of fibers conveying auditory information from the thalamus to the cortex. Topographical knowledge of this bundle is scarce and its diffusion-based tractographic reconstruction remains hardly achievable, especially for commonly available MRI acquisition protocols. In this scenario, validation of tractography results is particularly important. In this study we used blunt dissection to precisely characterize AR topography and relationships with adjacent bundles. Being aware of the anatomical characteristics of the tract provides us with the underlying ground truth on which methodological decisions, aimed at overcoming the limits of the tractographic reconstruction, can be made.