We describe a model-free method to quantify the spectrum of water mobilities in fixed and live brain tissues. We eliminate confounds caused by anisotropic diffusion in brain tissues by measuring orientationally-averaged diffusion weighted images over a large range of b-values. Spectra of orientationally-averaged water diffusivities show clear distinctions between white matter, gray matter and cerebrospinal fluid, and could provide new biologically-specific clinical markers for studying and diagnosing ischemic stroke, tumors, and neurodegenerative disorders and diseases, including inflammation.
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