The most promising techniques for understanding the biophysical basis of the MRI signals rely on combining multi-modal quantitative maps as a source of spatially-resolved information. If the MRI maps are not obtained from simultaneous acquisitions, they need to be co-registered in order to ensure a consistent localization of the signal sources for pixel-by-pixel fitting. However, the effects of the co-registration step are notoriously difficult to quantify. Here, we present a method for investigating the bias associated with co-registration for different quantitative MRI acquisitions, and outline the relevance of these biases for multi-parameter analysis.
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