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Abstract #1775

Detection of abnormal brain neural circuits in draxin knockout mice using DTI-MRI

Yuri Kitamoto1, Tatsuya Higuma1, Makoto Hirakane1, Mitsuhiro Takeda1, Sosuke Yoshinaga1, Rikita Araki2, Hideaki Tanaka1, Yohei Shinmyo3, and Hiroaki Terasawa1

1Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan, 2Bruker BioSpin K.K., Yokohama, Japan, 3Graduate School of Medical Sciences, Kanazawa University, Ishikawa, Japan

The aim of this study is to clarify the role of the axon guidance molecule Draxin in the construction of brain neural circuits by DTI-MRI. MRI was performed on draxin knockout, dra(–), and normal mice both in vivo and ex vivo. The in vivo study of dra(–) revealed that the nerve fibers in the corpus callosum did not intersect with the midline. The ex vivo study of dra(–) demonstrated that the thalamocortical nerve fibers did not extend toward the cerebral neocortex through the internal capsule. We successfully evaluated the influences caused by the Draxin loss with DTI-MRI.

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