Measuring the arterial transit time (ATT) helps for the optimization and quantification of arterial spin labeling experiments. Moreover, ATT may provide information on potential underlying vascular pathologies. In preclinical perfusion studies, multiple 2D-slices are commonly acquired to measure perfusion. However, this readout limits the number of slices for which ATT can be measured accurately in rodents. In this study, we implemented and optimized a dynamic ASL labeling scheme with a 3D echo planar imaging (EPI) readout to simultaneously map cerebral blood flow, arterial transit time and tissue T1 in the mouse brain at 9.4T.