Pseudo-continuous arterial spin labelling is regarded as the gold standard for clinical ASL, and can be improved in humans using multiphase sequences, but has not previously been implemented in mice. A multiphase pseudo-continuous ASL sequence to measure cerebral blood flow in mice was successfully implemented using respiratory triggering and optimisation of imaging readout, tag placement, labelling bolus duration, and post-label delay. Multiphase pseudo-continuous ASL is sensitive to changes in perfusion in an intracerebral glioma model.
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