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Abstract #1892

Combined 3D perfusion and diffusion MRI to phenotype the mouse brain: evaluation and application to a model of schizophrenia

Ivy Uszynski1,2, Lydiane Hirschler1,3, Jan M. Warnking1,2, Cyril Poupon4, Jean-Christophe Deloulme1,2, and Emmanuel L. Barbier1,2

1Grenoble Institut des Neurosciences, Université Grenoble Alpes, Grenoble, France, 2INSERM U1216, Grenoble, France, 3Bruker Biospin, Ettlingen, Germany, 4NeuroSpin, CEA Saclay, Gif-sur-Yvette, France

Perfusion and diffusion imaging both represent powerful tools in order to phenotype mouse models of brain diseases. Indeed, imaging cerebral blood flow (CBF) may be seen as a surrogate marker of brain metabolism while diffusion imaging provides the structural aspects of brain wiring. To evaluate the potential of combined CBF/Diffusion phenotyping, we evaluated the effect of knocking-out (KO) the microtubule-associated protein 6 (MAP6), which plays a critical role during the development of cerebral axonal tracts. Experiments were performed on homogeneous C57Bl6/129Sv mice using 3D pseudo-continuous Arterial Spin Labeling (pCASL) and 3D diffusion tensor imaging (DTI) at 9.4T.

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