With the rise in obesity globally, there is great interest in quantifying body composition and in particular metabolically active visceral adipose tissue (VAT). Increased accumulation of VAT has been linked with an increase in risk factors for cerebrovascular disease and Type 2 Diabetes (T2DM) . We investigated the degree to which weight, subcutaneous fat visceral fat, liver and lipid markers are affected by 2 weeks of overfeeding at 25% excess energy given as either carbohydrate or fat. 2 weeks of 25% excess energy overfeeding of either carbohydrate of fat does not alter subcutaneous or visceral abdominal fat in line with no changes in weight. MRI measurements of SAT and VAT can be used in longitudinal studies of diet to ascertain any changes in abdominal body fat deposition.