This study investigated the merit of liver T1rho values for detecting fibrosis in patients with chronic hepatitis B and the potential impact of fatty liver on T1rho measurements. Eighteen healthy control subjects, eighteen patients with clinically diagnosed simple fatty and eighteen with liver fibrosis were underwent T1rho MRI and mDIXON-Quant. Mean T1rho values and fat fraction (FF) were compared among the three groups. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the merit of T1rho values for detecting liver fibrosis. T1rho values were correlated with FF and clinical data. Our results showed significant differences in T1rho values among the three groups and T1rho had moderate diagnostic efficacy to detect fibrosis. T1rho values were not correlated with FF, subject age, or body mass index. We conclude liver T1rho values can be used as a MR biomarker for liver fibrosis and are not influenced by fatty liver severity.