Myopia is a major cause of blindness worldwide. 134 patients with high myopia and 120 emmetropic volunteers were examined. All of the participants were scanned by high resolution 3D T2-weighted CUBE MRI sequence. This study is to evaluate the preoperative localization and postoperative assessment value of high-resolution 3D MRI for high myopia. It is shown that the axial length, horizontal length, vertical length and volume of the high myopia were significantly longer than those of the emmetropia. And high resolution 3D MRI could clearly demonstrate the position of staphylomas, superior rectus, inferior rectus, medial rectus, lateral rectus and optic nerve.