There is increasing evidence that neurovascular dysregulation contributes to the persistent symptoms in patients with traumatic brain injury (TBI). Damaged microvasculature may disrupt the neurovascular coupling, where the local cerebral blood flow (CBF) no longer matches the metabolic requirements of the tissue. In the present study, we found that there was a diffuse hypoperfusion at thalamus, posterior cingulate gyrus, precuneus and white matter in 2-week follow-up scan relative to the 48-hour initial and 3-month follow-up scans. Low perfusion was sustained in both frontal and parietal cortices in 3-month follow-up scan. Our perfusion findings in these brain regions suggest that hypoperfusion may play a role in the post-traumatic symptoms of moderate TBI.