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Abstract #2941

Measuring lactate dehydrogenase activity with proton detected 13C hyperpolarization

Felix Kreis1, Jiazheng Wang1, Alan Wright1, and Kevin Brindle1

1Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, United Kingdom

Dissolution Dynamic Nuclear Polarized NMR (dDNP NMR) is a promising new tool for assessing metabolism in vivo. The signals of the hyperpolarized substrate and its downstream metabolites are usually detected by direct 13C observation. Here we demonstrate an effective way to repetitively transfer hyperpolarization via indirect couplings from [1-13C] to [3,3,3-1H3] in [1-13C] lactate formed from hyperpolarized [1-13C] pyruvate. The changes in the hyperpolarized [3,3,3-1H3] lactate peak were fitted to a kinetic model. The method sets the stage for dynamic hyperpolarized 1H imaging.

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