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Abstract #2985

Experimental setup for direct observation of hippocampal glycolysis in rat brain as a marker of cellular health

Dirk Ernst Johannes Cleppien1, Bernd Lecher2, Christian Kempe2, Markus Sack1, Anja Meier2, Max Kullack2, Wolfgang Weber-Fahr1, and Alexander Sartorius1

1RG Translational Imaging, Department NeuroImaging, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany, 2mfd diagnostics GmbH, Wendelsheim, Germany

Glycolysis is fundamental for cerebral energy metabolism. The current glycolysis pathway gives direct information about the local cellular health. To investigate hippocampal glycolysis in living rats, we established an experimental setup combining MRS and laser spectroscopy. It provides the opportunity to observe the behavior to the involved substances (glucose, lactate, NADH) for different glycolytic pathways. Under hyperglycemic conditions, blood oxygen limits glucose consumption leading to drops in NADH and lactate. In contrast, NADH and lactate concentrations increase under anaerobic conditions. This corresponds well with known literature and demonstrates the power of the presented setup to characterize hippocampal glycolysis in vivo.

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