In this study, we aim to reduce the bias inherent in fitting short echo time spectra by performing a two-point saturation recovery experiment. At both points lipid and macromolecule magnetisation is excited from an approximately fully relaxed state and so this signal component is unchanged, thus the fitting model can be constrained. We show that this may be an advantageous strategy in comparison to other common techniques, reducing bias where fitting spectra with narrow linewidths.
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