Abstract #3029

Longitudinal Investigation of the Metabolome of Developing 3D Aggregating Brain Cell Cultures at Different Maturation Stages by 1H HR-MAS NMR

Gaelle Diserens¹, Martina Vermathen², Chiara Sartori^3,4, Marie-Gabrielle Zurich^3,4, and Peter Vermathen¹

¹Depts. Radiology and Clinical Research, University Bern, Bern, Switzerland, ²Dept. Chemistry and Biochemistry, University Bern, Bern, Switzerland, ³Dept. Physiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland, ⁴Swiss Center for Applied Human Toxicology (SCAHT), Switzerland

3D rat brain aggregate model is an excellent tool for mechanistic studies including OMICS analysis. However, their metabolic profile have not been yet fully investigated. The aim was to investigate by ¹H-HR-MAS NMR the metabolic fingerprint of 3D brain cell cultures at different maturation stages to establish a developmental profile of metabolic changes. Chemometric analysis revealed a clear separation of samples from the different maturation days. Metabolite concentration evolutions could be followed and revealed strong and various metabolic alterations. The strong metabolite evolution emphasizes the brain modelling complexity during maturation, possibly reflecting physiological processes of brain tissue development.

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