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Abstract #3028

PROTON NMR METABOLIC PROFILING OF CSF REVEALS DISTINCT DIFFERENTIATION OF MENINGITIS WITH NEGATIVE CONTROLS

Tanushri Chatterji1, Suruchi Singh2, Ajai Kumar Singh3, Manodeep Sen4, and Raja Roy2

1Departent of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, India, 2Centre of Biomedical Research, Lucknow, India, 3Department of Neurology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, India, 4Department of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow

The chemical composition of cerebrospinal fluid (CSF) in central nervous system (CNS), varies during onset of meningitis, neurodegenerative disorders and in traumatic cases.The study attempted to observe the metabolic variation in meningitis cases, negative controls and positive controls. Further differentiation among the groups was carried out using Principal Component Analysis (PCA) followed by Partial Least Square Discriminant Analysis (PLS-DA).On the basis of metabolic profile it was found that negative control CSF samples are more appropriate for differentiation of meningitis than positive control CSF samples.The biomarkers identified were ketone bodies, amino acids, propylene glycol, citrate and creatine/creatinine.

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