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Abstract #3027

Towards Imaging the Glycolytic and Glutaminolytic Differences in Prostate Cancer Cell Lines that Affects Outcome of Glutaminase Inhibition

Niki Zacharias Millward 1,2, Christopher McCullough3, Sriram Shanmugavelandy1, Jaehyuk Lee1, Youngbok Lee4, James McHenry5, Lawrence Jones 6, and Pratip Bhattacharya7

1Cancer Systems Imaging, MD Anderson Cancer Center, Houston, TX, United States, 2Bioengineering, Rice University, Houston, TX, United States, 3Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology, Rockville, MD, United States, 4Applied Chemistry, Hanyang University, Korea, Republic of, 5University of Houston Downtown Campus, Houston, TX, 6Huntington Medical Research Institutes, Pasadena, CA, 7MD Anderson Cancer Center, Houston, TX, United States

To understand and image how metabolism changes in prostate cancer (PCa), we determined both extracellular and intracellular metabolic profile of four PCa cell lines with varying degrees of aggressiveness. Differences in metabolism and mechanistic link were further explored using carbon-13 glucose and glutamine feeding studies and hyperpolarized pyruvate metabolic imaging trials with subcutaneous xenograft PC3 and PC3M animal models. We found increased glutamine utilization in the more metastatic cell line PC3M and this increased dependence on glutamine leads to reduction in cell proliferation and ATP when cells are treated with glutaminase inhibitor CB-839. No reduction is seen in PC3 line.

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