To understand and image how metabolism changes in prostate cancer (PCa), we determined both extracellular and intracellular metabolic profile of four PCa cell lines with varying degrees of aggressiveness. Differences in metabolism and mechanistic link were further explored using carbon-13 glucose and glutamine feeding studies and hyperpolarized pyruvate metabolic imaging trials with subcutaneous xenograft PC3 and PC3M animal models. We found increased glutamine utilization in the more metastatic cell line PC3M and this increased dependence on glutamine leads to reduction in cell proliferation and ATP when cells are treated with glutaminase inhibitor CB-839. No reduction is seen in PC3 line.
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