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Abstract #3052

Mn2+ uptake mechanisms in organotypic rat hippocampal slice cultures

Alexia Daoust1, Galit Saar1, Steven Dodd1, and Alan Koretsky1

1National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, United States

MEMRI can be used for different applications such as tracing neuronal connections or functional imaging. However, Mn2+ uptake and transport mechanisms are still unclear. These mechanisms were studied in an organotypic hippocampal slice culture. After adding Mn to the medium, MRI contrast changes were affected by Ca channel manipulation and other metals that use divalent metal transporters. Mn cellular uptake was also affected by both, the presence of glutamate receptor antagonist and a neuronal activity inhibitor. Our results establish hippocampal slice as a system to study the cellular mechanisms related to MEMRI.

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