Abstract #3082

Study of the Tetracycline-controlled Transcriptional Activation of c-Myc in Burkitt Lymphoma B-cell Line P493-6 Using Hyperpolarized [1-13C]pyruvate

Eugen Kubala^1,2,3, Laura Jacobs⁴, Julia Kempf⁴, Kim A Muñoz Alvarez¹, Rolf F Schulte³, Steffen J Glaser², Markus Schwaiger¹, Marion I Menzel³, and Ulrich Keller⁴

¹Department of Nuclear Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany, ²Department of Chemistry, Technische Universität München, Munich, Germany, ³GE Global Research, Munich, Germany, ⁴III. Medical Department, Klinikum rechts der Isar, Technische Universität München, Munich, Germany

We proved that the transcriptional activation of c-Myc expression controlled by tetracycline has a direct influence on lactate dehydrogenase-A (LDH-A) activity in B-cell line P493-6 in vitro. Using hyperpolarized [1-¹³C]pyruvate and ¹³C magnetic resonance spectroscopy we were able to monitor a reduction of pyruvate to lactate reaction catalyzed by LDH-A. Incubation of the P493-6 cells in a media with 0.1 μg/mL tetracycline for 24 hours reduced the kinetic value of the reaction by 41.8±10.5 %. This proves that the control of c-myc has a significant influence on LDH-A activity and can be measured using ¹³C magnetic resonance spectroscopy with hyperpolarized [1-¹³C]pyruvate.

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