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Abstract #3082

Study of the Tetracycline-controlled Transcriptional Activation of c-Myc in Burkitt Lymphoma B-cell Line P493-6 Using Hyperpolarized [1-13C]pyruvate

Eugen Kubala1,2,3, Laura Jacobs4, Julia Kempf4, Kim A Muñoz Alvarez1, Rolf F Schulte3, Steffen J Glaser2, Markus Schwaiger1, Marion I Menzel3, and Ulrich Keller4

1Department of Nuclear Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany, 2Department of Chemistry, Technische Universität München, Munich, Germany, 3GE Global Research, Munich, Germany, 4III. Medical Department, Klinikum rechts der Isar, Technische Universität München, Munich, Germany

We proved that the transcriptional activation of c-Myc expression controlled by tetracycline has a direct influence on lactate dehydrogenase-A (LDH-A) activity in B-cell line P493-6 in vitro. Using hyperpolarized [1-13C]pyruvate and 13C magnetic resonance spectroscopy we were able to monitor a reduction of pyruvate to lactate reaction catalyzed by LDH-A. Incubation of the P493-6 cells in a media with 0.1 μg/mL tetracycline for 24 hours reduced the kinetic value of the reaction by 41.8±10.5 %. This proves that the control of c-myc has a significant influence on LDH-A activity and can be measured using 13C magnetic resonance spectroscopy with hyperpolarized [1-13C]pyruvate.

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