Meeting Banner
Abstract #3081

Evaluation of the in vivo on-target effect of a newly developed LDH inhibitor using hyperpolarized 13C Magnetic Resonance Spectroscopic Imaging

Nobu Oshima1, Shun Kishimoto2, Keita Saito2, Dan Crooks3, Kristin Beebe3, Kazutoshi Yamamoto2, Jeffery Brender2, Ganesha Rai4, Bryan T Mott4, David J Maloney4, James B Mitchell2, Murali K Cherukuri2, and Leonard M Neckers3

1RBB,UOB/NCI, NIH, Bethesda, MD, United States, 2RBB/NCI, NIH, Bethesda, MD, United States, 3UOB/NCI, NIH, Bethesda, MD, United States, 4National Center for Advancing Translational Sciences (NCATS), NIH, Rockville, MD, United States

This study aimed to monitor the impact on metabolic flux in vivo of a newly developed Lactate Dehydrogenase A Inhibitor (LDHI), using hyperpolarized 13C Magnetic Resonance (MR) technology. Using hyperpolarized 13C MR Spectroscopy and Chemical Shift imaging, we found that the LDHI significantly and rapidly suppressed the [1-13C]lactate to [1-13C]pyruvate ratio after single dose administration to mice harboring a MiaPaca (a glycolytic pancreatic cancer cell line) xenograft. These results indicate that the LDHI suppressed lactate production in the tumors. Thus, using Hyperpolarized 13C MRI provides a very useful technology to evaluate in vivo on-target efficacy of LDH inhibitors.

This abstract and the presentation materials are available to members only; a login is required.

Join Here