Renal IRI is a leading cause of AKI in several disease states; there is a current lack of precise methods to directly assess success of kidney transplant after reperfusion. We here showed how we can measure metabolic function in both the contralateral kidney and post-ischemic 2 min after reperfusion and again after 1 hour of reperfusion. We here saw a very different response compared to metabolic data collected from animals after 24 hours of reperfusion. In this study we induced mild/moderate ischema and it looks like we captured metabolic images in a phase of repair and salvage to maintain normal kidney filtration function in the animals. It seems that severe injury has not yet occurred, or maybe won’t occur. Together with perfusion measurements or kidney filtration measurements this method might hold some clinical value.