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Abstract #3273

The impact of kinetic isotope effects in using deuterated glucose for metabolic experiments

Alexander Funk1, Brian Anderson1, Xiaodong Wen1, Chalermchai Khemtong1, Dean Sherry1,2, and Craig Malloy1

1Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas, TX, United States, 2University of Texas at Dallas

Deuteration of 13C-enriched glucose has been used to prolong T1 of hyperpolarized 13C with the objective of monitoring glucose metabolism to lactate. However, the effect of deuteration on the flux in glycolysis in vivo has not been investigated extensively. Deuterated glucose was studied in perfused hearts and extracts were analyzed for a deuterium kinetic isotope effect using 1H, 13C and 2H NMR spectroscopy. Unexpectedly, there was extensive exchange of methyl deuterons in lactate with aqueous medium. Perdeuteration slows metabolism of glucose to lactate.

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