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Abstract #3600

Post-mortem Cardiac and Skeletal Muscle 19F MRI of PFCE-labeled and FuGENE-transfected Cardiac Progenitor Stem Cells in the C57BL/6 Mouse

Christakis Constantinides1, Ricardo Carnicer1, Ayman Al Haj Zen1, Mahon Maguire1, Eileen McNeil1, Edyta Swider2, Mangala Srinivas2, Carolyn A Carr3, and Jurgen E Schneider1

1Cardiovascular Medicine, University of Oxford, Oxford, United Kingdom, 2Department of Tumor Immunology, Radboud University, Nijmegen, Netherlands, 3Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom

Stem cell (SC) technologies constitute a potential new therapeutic approach aiming to achieve tissue regeneration. Despite advances in the visualization of pre-labeled SCs with SPIOs (1H MRI), and of nanoparticles (NPs) containing perfluoro-crown-ethers (PFCE) [2-4] in 19F MRI, there have been no prior reports on cardiac 19F imaging with direct SC injections. We report herein the implementation of a fast acquisition protocol for cardiac and skeletal muscle 19F imaging of the C57BL/6 mouse post-mortem, and identify the minimum cellular load for PFCE labels to achieve visualization following direct SC cell injections.

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