Abstract #3600

Post-mortem Cardiac and Skeletal Muscle 19F MRI of PFCE-labeled and FuGENE-transfected Cardiac Progenitor Stem Cells in the C57BL/6 Mouse

Christakis Constantinides¹, Ricardo Carnicer¹, Ayman Al Haj Zen¹, Mahon Maguire¹, Eileen McNeil¹, Edyta Swider², Mangala Srinivas², Carolyn A Carr³, and Jurgen E Schneider¹

¹Cardiovascular Medicine, University of Oxford, Oxford, United Kingdom, ²Department of Tumor Immunology, Radboud University, Nijmegen, Netherlands, ³Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom

Stem cell (SC) technologies constitute a potential new therapeutic approach aiming to achieve tissue regeneration. Despite advances in the visualization of pre-labeled SCs with SPIOs (¹H MRI), and of nanoparticles (NPs) containing perfluoro-crown-ethers (PFCE) [2-4] in ¹⁹F MRI, there have been no prior reports on cardiac ¹⁹F imaging with direct SC injections. We report herein the implementation of a fast acquisition protocol for cardiac and skeletal muscle ¹⁹F imaging of the C57BL/6 mouse post-mortem, and identify the minimum cellular load for PFCE labels to achieve visualization following direct SC cell injections.

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