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Abstract #3742

Assessment of hepatic glycogen metabolism ex vivo and in vivo in mice using chemical exchange saturation transfer MRI

Corin Miller1, Jin Cao1, Chunlian Zhang1, Eduard Chekmenev2, Bruce Damon2, Alan Cherrington3, and John Gore2

1Translational Imaging Biomarkers, Merck & Co., Inc, West Point, PA, United States, 2VUIIS, Vanderbilt University, Nashville, TN, United States, 3Vanderbilt Medical Center, Vanderbilt University, Nashville, TN, United States

Despite being integral to whole body glucose homeostasis, liver glycogen remains difficult to measure non-invasively. Recent works have demonstrated the feasibility of detecting liver glycogen using CEST MRI. In this presentation we present data that builds upon this observation and investigate whether CEST can be used to monitor glycogen synthesis and breakdown in mice in real time both ex vivo in a perfused liver system, and in vivo. Treatment with hyperglycemia or glucagon resulted in increases or decreases, respectively, in the CEST MTRasym AUC over time. This demonstrates that CEST-based approaches can be used to non-invasively monitor glycogen metabolism.

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