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Abstract #4071

MEMRI atlas-based assessment of brain volumes in adult NSG mice irradiated at birth

Balasrinivasa R Sajja1, Aditya N Bade2, Mariano G Uberti1, Michael D Boska1,2, Howard E Gendelman2, Santhi Gorantla2, and Yutong Liu1,2

1Radiology, University of Nebraska Medical Center, Omaha, NE, United States, 2Pharmacology & Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, United States

Use of HIV-1 humanized mice allows the evaluation of brain morphology affected by disease and disease altering therapies. We posit that such studies can elucidate the pathobiology of HIV-1 associated neurological disorders. As irradiation, administered at birth, is a required step for long-term graft integrity we evaluated the effect of irradiation on mouse brain development using manganese-enhanced MRI (MEMRI) atlas-based segmentation. Brain size reductions in irradiated mice showed substantive morphological alterations. Thus, evaluation of irradiation effects on brain morphology is a requisite step in assessments of virus-induced brain pathology.

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