The environment of the brain dynamically changes with neonatal brain development, and the topic of whether the application of magnetic resonance imaging (MRI) can reflect these brain environment changes is a recent one. In recent years, a new magnetic resonance contrast technology called amide proton transfer (APT) imaging has emerged which can detect protein and peptides through the signal from water (1), reflecting in vivo pH and protein concentration at the cellular and molecular level. The so-called amide proton mainly refers to the amide proton from the free protein and the polypeptide backbones.
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