The relationship between tissue microstructure and ADC is poorly understood in cancer. We identified quantitative nuclear characteristics from haematoxylin, eosin, and saffron stained prostatic histology samples. ADC maps were calculated for transgenic prostate cancer mice and healthy controls. Matched histology images of excised prostates were used to determine cellularity and histological class. ADC and cellularity correlated with a set of nuclear features (describing texture, lightness, radius, and shape), which exhibited mirrored trends, implying an inverse relationship between ADC and cellularity. The same nuclear features correlated with histological class. This will be useful for underpinning relationships between ADC and tissue microstructure.