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Abstract #4491

Radiological comparisons of 3D and 2D-cell culture derived tumours in an orthotopic mouse xenograft model of paediatric glioblastoma

Mariama Fofana1, Jessica KR Boult1, Maria Vinci1, Valeria Molinari1, Sergey Popov1, Alan Mackay1, Angel M Carcaboso2, Chris Jones1, and Simon P Robinson1

1The Institute of Cancer Research, London, United Kingdom, 2Department of Oncology, Hospital Sant Joan de Déu, Barcelona, Spain

Primary patient-derived paediatric glioblastoma (pGBM) cells, cultured under conditions designed to maintain stem-like phenotypes, provide valuable platforms for pre-clinical research. Culturing tumour cells in suspension (3D) or adherent on laminin (2D) may influence their growth behaviour in vivo. Longitudinal, anatomical MRI showed that orthotopic HSJD-GBM-001 xenografts derived from 3D-cultured cells grew significantly faster than those from 2D-culture. Infiltrative tumour growth, maintenance of the blood brain barrier and high ADC, T1 and T2, associated with histopathologically confirmed tumour-oedema, were observed in both cohorts. The study highlights the influence of in vitro cell culture conditions on in vivo tumour growth characteristics.

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