Prospective gating and automatic reacquisition of data corrupted by respiration motion were implemented in 3D balanced steady-state free precession (bSSFP) to provide contrast that enables unambiguous detection of liver tumours in the whole mouse liver with 200 µm isotropic resolution and in scan times that are routinely less than 7 minutes. The method was used for orthotopic tumour burden quantification in 8 female C57BL/6 mice at days 7, 9, and 11 post intra-hepatic injection of MC38-GFP cells, and enabled measurement of tumour volumes less than 1 mm3.
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