To validate to what extent the fiber orientations derived from diffusion MRI (dMRI) reveal anatomical reality, polarized light imaging (PLI) allowing the fiber orientations of the human heart to be physically measured with high spatial resolution was used. The dMRI and PLI orientation measurements of the same hearts are then compared using a multimodal registration-based framework. Experimental results show that dMRI and PLI have similar variation patterns of elevation or azimuth angles, except that dMRI introduced a decrease of about 24º in transmural elevation angle range. No significant differences were observed on azimuth angle in both modalities.
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