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Abstract #5000

MRI/S Assessment of Skeletal Muscle Morphology and Energetics in Mdx Muscle Injured Mouse as a Model for Duchenne Muscular Dystrophy

HASAN ALSAID1, Mary Rambo1, Tinamarie Skedzielewski1, Alan McDougal2, Fritz Kramer2, and Beat Jucker1

1Bioimaging, IV/IVT, PTS, GlaxoSmithKline, King of Prussia, PA, United States, 2Muscle Metabolism DPU, MPC TAU, GlaxoSmithKline, King of Prussia, PA, United States

The purpose of this study was to longitudinally and non-invasively assess the effect of eccentric contraction induced muscle damage in the Mdx mouse as a model for Duchenne Muscular Dystrophy using non invasive MRI and MRS. Mdx mice showed a significant increase in absolute T2 value at baseline and a severe increase in the exercised leg at Day 2 following injury compared to the Wild type group. PCr/Pi ratios decreased in the Mdx group acutely upon exercise induced damage and resolved by day 7. The fraction of Undamaged Limb Force is correlated negatively with T2 and positevely with the PCr/Pi ratios.

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