Venous oxygenation level reflects the cerebral metabolic rate of oxygen and its measurement facilitates studies of animal models of diseases. We developed a non-invasive and non-contrast-agent method based on T2-Relaxation-Under-Spin-Tagging (TRUST) to quantify cerebral venous oxygenation in mice at 11.7T. A series of studies were performed to optimize key imaging parameters. This method may prove useful in studies of brain physiology and pathophysiology in animal models.
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