Quantitative measurement of oxygen delivery and consumption in the brain can provide insight into neurovascular and metabolic coupling. A single shot asymmetric spin echo (ASE) EPI sequence was implemented for quantitative oxygen extraction fraction (OEF) measurement in healthy volunteers for the feasibility study. OEF results were in good agreement with the normal range of 30%-40% reported in the previous literature. ASE EPI shows the potential to provide quantitative OEF maps with good brain coverage and without the need of gas challenges. Further investigation is required to evaluate the sensitivity of OEF measurement in the disease situation, such as stroke.