Resting state fMRI (rs-fMRI) used to detect and evaluate resting state functional connectivity (RSFC) in both healthy subjects and patients. However, the reproducibility of rs-fMRI may be influenced by the time of day when the scan is performed. In this work, we investigated the reproducibility of resting state networks by comparing scans performed in the morning (immediately after the scanner was switched on) and again in the late afternoon (after all daily scans were done) on six different days. Our results showed higher RSFC in afternoon scans in 5 regions within 5 networks .
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