Abstract #5468

Quantitative analysis of in vivo MEGA-PRESS spectra in mice at 9.4T

Jia Guo¹, Yanping Sun², Zhu Gang³, Scott A. Small⁴, and Douglas L. Rothman⁵

¹Department of Biomedical Engineering, Columbia University, New York, NY, United States, ²Department of Medicine, Columbia University, New York, NY, ³Bruker BioSpin, Billerica, MA, ⁴Departments of Neurology, Radiology or Psychiatry, Columbia University, New York, NY, ⁵Departments of Biomedical Engineering or Radiology and Biomedical Imaging, Yale University, New Haven, CT

As of yet J-difference editing methods such as MEGA-PRESS have not been applied to in vivo mouse studies due to low sensitivity and the lack of an automated program to analyze the data. To overcome the obstacles, a fully automated software toolkit MRSMouse was developed for the quantitative analysis of MEGA-PRESS data of the mouse brain. With MRSMouse, we demonstrated the feasibility and reproducibility of MEGA-PRESS for detection of GABA in the mouse brain using a Bruker BioSpec 94/20 9.4T system. During anesthesia, significant decreases of mouse thalamic GABA levels caused by isoflurane were noticed in vivo for the first time.

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