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Abstract #5594

In vivo brain redox status and blood-brain barrier function in diethylmaleate-treated mice by EPR imaging and ME-MRI

Hirotada G Fujii1, Miho C Emoto1, Yuta Matsuoka2, and Ken-ichi Yamada2

1Sapporo Medical University, Sapporo, Japan, 2Kyushu University, Fukuoka, Japan

EPR imaging has been used to visualize redox status in oxidative brain diseases, but the role of cerebral glutathione (GSH) is not clear. In this study, using the mouse model of GSH depletion with diethylmaleate (DEM), the role of GSH in brain redox status was examined. The remarkable change in redox status in DEM-treated mouse brain was visualized with EPR imaging, and in vitro assay showed decrease in the level of GSH. ME-MRI clearly visualized blood-brain barrier dysfunction in DEM-treated mice. Results indicate that GSH plays an important role in the maintenance of both brain redox status and BBB integrity.

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