Cachexia is a poorly understood metabolic syndrome characterized by cancer-induced tissue wasting and weight loss. Cachexia occurs with the highest frequency and severity in pancreatic ductal adenocarcinoma (PDAC). To further understand this syndrome, here we used 1H MRS to analyze liver metabolites in mice with and without cachexia-inducing PDAC. We detected profound liver weight loss in cachectic mice. 1H MR spectra identified significant depletion of lactate, glucose and glutathione in cachectic mice that provide new insights into the syndrome and may present novel strategies to prevent or reduce cachexia-induced weight loss and the morbidity and mortality associated with the syndrome.