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Abstract #5626

Monitoring of cell proliferation by 19F-MRS via quantitation of 3'-deoxy-3'-fluorothymidine (FLT) and its monophosphate metabolite (FLT-MP) in invivo

Inok Ko1, Ki-Hye Jung1, Kyung Jun Kang1, Kyo Chul Lee1, Yong Jin Lee1, Jung-Young Kim1, Sang Moo Lim2, and Ji-Ae Park1

1Division of RI-Convergence Research, Korea Institute Radiological and Medical Sciences, Seoul, Korea, Republic of, 2Department of Nuclear Medicine, Korea Institute Radiological and Medical Sciences, Seoul, Korea, Republic of

Distinguishing between FLT(fluorothymidine) and FLT-MP(flruorothymidine - monophosphate) by imaging methods is important for evaluating the tumor cell proliferation rate. The aim of this study is to develop and validate a suitable 19F MR Spectroscopy for measuring TK1 activity via quantitation of FLT and FLT-MP in vivo. We observed the good correlations between SNR and FLT concentration (r2= 0.94). In phantom study, the locations of FLT and FLT-MP was -175.99, -175.24 ppm, respectively. In vivo study, FLT spectrum in mouse tumor was observed in 25 min after injection, whereas FLT-MP spectrum occurred in 90 min after injection. This result shows that 19F MR Spectroscopy is suitable for monitoring of FLT-MP generation in in vivo.

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