Meeting Banner
Abstract #0107

In-vivo metabolism of co-hyperpolarized [1-13C] pyruvate and [1,3-13C] acetoacetate identifies cytosolic and mitochondrial redox in ischemic perfused hearts

Gaurav Sharma1, Craig R. Malloy1,2,3, A. Dean Sherry1,2,4, and Chalermchai Khemtong1,2

1Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX, United States, 2Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX, United States, 3Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, United States, 4Department of Chemistry, University of Texas at Dallas, Dallas, TX, United States

Cellular redox state is intricately linked with cardiac ischemia. Historically, tissue levels of lactate to pyruvate and β-hydroxybutyrate to acetoacetate have been used as indices of cytosolic and mitochondrial redox, respectively. The present study was designed to evaluate the potential of using co-hyperpolarized (HP) [1-13C] pyruvate and [1,3-13C] acetoacetate as reporters of cytosolic and mitochondrial redox, respectively. 13C NMR spectra of perfused rat hearts displayed increased production of both HP-lactate and HP-β-hydroxybutyrate in low flow ischemia, rotenone, and aminooxyacetate (AOA) treated hearts. The result suggests that HP-lactate and HP-β-hydroxybutyrate are sensitive metabolic markers of tissue redox in heart tissue.

This abstract and the presentation materials are available to members only; a login is required.

Join Here