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Abstract #0281

Detection of D-amino acid oxidase using hyperpolarized molecular probes

Alice Radaelli1, Hikari Ananda Infinity Yoshihara1, and Rolf Gruetter1,2

1Laboratory for Functional and Metabolic Imaging (LIFMET), EPFL, Lausanne, Switzerland, 2Center for Biomedical Imaging (CIBM), Lausanne, Switzerland

D-amino acid oxidase (DAO) is an enzyme that catalyzes the degradation of D-amino acids in the body. Here, we explored the possibility of detecting D-amino acid oxidase activity by monitoring its metabolism in the rat kidney after a bolus injection of hyperpolarized D-[1-13C]alanine. Our data show that D-alanine is readily converted to lactate only when the DAO enzyme is not inhibited, indicating that the observed metabolism is that of DAO.

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