Abstract #0281

Detection of D-amino acid oxidase using hyperpolarized molecular probes

Alice Radaelli¹, Hikari Ananda Infinity Yoshihara¹, and Rolf Gruetter^1,2

¹Laboratory for Functional and Metabolic Imaging (LIFMET), EPFL, Lausanne, Switzerland, ²Center for Biomedical Imaging (CIBM), Lausanne, Switzerland

D-amino acid oxidase (DAO) is an enzyme that catalyzes the degradation of D-amino acids in the body. Here, we explored the possibility of detecting D-amino acid oxidase activity by monitoring its metabolism in the rat kidney after a bolus injection of hyperpolarized D-[1-¹³C]alanine. Our data show that D-alanine is readily converted to lactate only when the DAO enzyme is not inhibited, indicating that the observed metabolism is that of DAO.

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